ANYL 438 |
| Much attention has been focused on the use of microfluidics to improve cell culture and manipulation by creating biomimetic microenvironments.(1) Cell-based assays in microfluidic systems will also benefit from decreased reagent consumption, smaller cell populations, higher parallelism and automation. However, to take full advantage of microfluidic systems as culture sizes decrease toward single cell assays, it becomes important to accurately control cell placement and attachment. We present here the generation of ordered cell arrays inside microfluidic devices using dielectrophoretic trapping of neural cells. Polyelectrolyte multilayers (PEMs) are used to treat the microfluidic device, rendering it amenable to cellular attachment. It was found that >85% of cells were immobilized as they flowed through the microfluidic device. Using PEM treatment, neural cells remained adherent in a line perpendicular to flow after the electrodes were no longer energized (Figure 1). 1. Beebe, D.;Folch, A. Lab on a Chip 2005, 5, 10-11.
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General Papers
1:30 PM-4:35 PM, Wednesday, 31 August 2005 Washington DC Convention Center -- 153, Oral
Sci-Mix
Division of Analytical Chemistry |
