ANYL 439 |
We have created a family of water-soluble block copolymers of acrylamide and N-alkyl acrylamides that can selectively remove proteins from DNA via microchannel electrophoresis. A series of acrylamide co-monomers with varying N-alkyl chain lengths (C4, C6, C6-6, C8) were synthesized and characterized. Capillary and chip electrophoresis of bovine serum albumin proteins in these media reveals that N,N-dihexyl and N-octyl acrylamide copolymers show the most significant protein adsorption. Additionally, these copolymers are a novel class of DNA separation media: “physically crosslinked” polymer networks, which could meet the demands of increased separation performance within the bioanalytical community. Single-molecule DNA imaging reveals a novel separation modality, resembling “inch-worm” movement, which we have termed “stationary entanglement coupling.” Physically crosslinked gels exhibit three distinct concentrations regimes that have dramatic consequences on DNA separation mechanism and improved DNA resolution. Physically crosslinked systems have advantages over both linear polymers and covalently linked crosslinked gels because of separation performance (or speed), while unlike chemically crosslinked gels, our physically crosslinked networks can be “broken” (reversibly) and loaded into microchannels. Using these media, several hundred basepairs of DNA have been sequenced in microfluidic devices in under 10 minutes, with high peak efficiencies and excellent results compared to matched-molar mass LPA. This represents at least a ten-fold increase in throughput with far less sample consumption then conventional capillary electrophoresis.
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General Papers
1:30 PM-4:35 PM, Wednesday, 31 August 2005 Washington DC Convention Center -- 153, Oral
Sci-Mix
Division of Analytical Chemistry |
