Chemometric methods applied to the assay of serum lipids

ANYL 140

Neil Purdie, npurdie@okstate.edu1, Lisa M Reilly, lisa.reilly@okstate.edu1, Barry Lavine, bklab@chem.okstate.edu1, and David Marais2. (1) Department of Chemistry, Oklahoma State University, 107 Physical Sciences, Stillwater, OK 74078, (2) Lipidology Division of the Department of Medicine and MRC Cape Heart Group, University of Cape Town, University of Cape Town Medical School, Faculty of Health Sciences, Observatory, South Africa
An accurate colorometric method has been developed to routinely measure serum levels of cholesterol and polyunsaturated fatty acids simultaneously. A characteristic color and absorbance spectrum for each analyte is generated by Friedel-Crafts acylations of alkene functional groups under ambient temperature conditions. In mixtures each lipid reacts independently of all the others. The only apparent interference is from heme from lysed blood cells. The complex multicomponent spectra that result from serum tests have been analyzed using the Generalized Standard Addition Method to measure concentrations for all of the unsaturated lipids. Changes in the proportional amounts of the lipids from subject to subject lead to a wide range of spectral patterns that correlate with lipid dysfunctions. Principal component analysis of the spectral data followed by cluster analysis has revealed a strong correlation between these patterns and established disease states. The procedure has the potential to be used for future risk prediction models.
 

General Papers
7:00 PM-9:00 PM, Sunday, 28 August 2005 Washington DC Convention Center -- Hall A, Poster

Division of Analytical Chemistry

The 230th ACS National Meeting, in Washington, DC, Aug 28-Sept 1, 2005