ANYL 176 |
| The focus of this work is on the development of a bead-based microchip sensor array that is suitable for the rapid detection of DNA oligonucleotides. Specifically, for biological applications, self-addressable polymer microsphere matrices are selectively arranged in wells etched in silicon wafers. These micro-cavities possess trans-wafer openings, which allow for both fluid flow through the microreactors/analysis chambers and optical access to the chemically sensitive microbeads. The incorporation of molecular beacons into the microbead platform offers a powerful tool towards a label-free detection of nucleic acid based targets of interest. In efforts to effectively detect and quantitate low amounts of target genomic material, a micromachined glass capillary chip was fabricated to provide a thermal amplication step prior to microarray detection. Collectively, these features allow for the identification and quantitation of target DNA analytes to occur in near-real-time using fluorescence changes that accompany the target sample introduction. Alternatively, the capture of short oligonucleotides with the microbead system affords hybridization times on the order of minutes with point mutation selectivity factors greater than 3,800 and limit of detection values of ~10-14 M.
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General Papers
7:00 PM-9:00 PM, Sunday, 28 August 2005 Washington DC Convention Center -- Hall A, Poster
Sci-Mix
Division of Analytical Chemistry |