ANYL 185 |
| Magnetic particles have been widely used as solid phases in immunoassays. Conventionally, antibodies are either covalently bonded or adsorbed to the polymeric coating of the solid phase, in which randomly oriented antibodies are immobilized, leading to heterogeneous binging constants. In this work, to achieve better orientation control of the immobilized antibodies, gold-coated magnetic particles are prepared and used as a solid phase for developing a sandwich type enzyme immunoassay to detect C-reactive protein. Gold-coated magnetic particles are prepared through an electroless plating method and characterized. Fab's fragments of a desired capture antibody population are immobilized on the gold-coated magnetic particles via a thiol-Au bond. It is shown that more active antibody is bound to the gold coated particles than conventional magnetic phases in which the same amount of total antibody binding sites are attached randomly. Results demonstrate that gold-coated magnetic particles provide enhanced antibody binding efficiencies and a lower detection limit for the measurement of C-reaction protein. |
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General Papers
7:00 PM-9:00 PM, Sunday, 28 August 2005 Washington DC Convention Center -- Hall A, Poster
Sci-Mix
Division of Analytical Chemistry |