Single molecular multi analyte fluorescent probes for bioimaging

ANYL 490

Hirokazu Komatsu, komatsu@applc.keio.ac.jp1, Takahiro Miki1, Yutaka Shindo2, Yoshiichiro Kitamura2, Kotaro Oka2, and Koji Suzuki, suzuki@applc.keio.ac.jp1. (1) Department of Applied Chemistry, Keio University, Hiyoshi 3-14-1, Kouhoku-ku, Yokohama, Japan, (2) Department of Biosciences and Informatics, Keio University, Hiyoshi 3-14-1, Kouhoku-ku, Yokohama, Japan
Multicolor-fluorescent imaging has been widely used for analysis of intracellular signal transmitters. We are proposing single molecular multi fluorescent probes; fluorescent probes for multiple analytes with different responses. They could overcome the problem from applying multiple indicators in a cell, such as spectral overlap, toxicity, localization, metabolism and photobleaching of the indicators. Intracellular calcium plays a critical role as signal transmitter and magnesium acts as a cofactor in many situations. We designed and synthesized a Ca2+-Mg2+ multi fluorescent probe KCM. KCM-1 showed a spectral blue shift with complexation to Ca2+ and a red shift to Mg2+. With ratiometric data analyse at different excitation wavelengths, the concentrations of Ca2+ and Mg2+ were simultaneously quantified. Applying AM-ester method, intracellular Ca2+ and Mg2+ were simultaneously imaged. With FCCP stimulation, Ca2+ and Mg2+ uptake from mitochondria was observed. The intracellular multiple analyte imaging by a single molecular multi fluorescent probe was firstly demonstrated.
 

General Papers
1:30 PM-5:00 PM, Thursday, 1 September 2005 Washington DC Convention Center -- 154B, Oral

Sci-Mix
8:00 PM-10:00 PM, Monday, 29 August 2005 Washington DC Convention Center -- Hall A, Sci-Mix

Division of Analytical Chemistry

The 230th ACS National Meeting, in Washington, DC, Aug 28-Sept 1, 2005