ANYL 411 |
| Detection of biological analytes in complex sample environments is often implemented at a solid-liquid interface. This talk will describe principles for label-free analysis being established using near-field optical methods in the GHz regime. Sensitivities comparable to fluorescence-based assay using conventional instrumentation, but without the need for any kind of analyte labeling, have been demonstrated. In parallel, we are developing microelectronic chips that serve as functional solid supports with integrated circuitry for control and detection of binding between surface-immobilized and free (analyte) species. By integration of the respective functions on-chip, these devices can replace macroscopic instrumentation at minimal cost by drawing on affordable CMOS manufacture. The design and validation of several prototypes for fluorescence-based measurements will be described, including designs with conventional optical filter-based approach as well as ones based on a time-resolved fluorescence (TFR) technique. Results from validation studies of these emerging technologies and their promise for more portable, simplified, and economical assays will be described. |
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Sensors and Instrumentation for Counterterrorism: Biological Agent Detection
1:30 PM-5:15 PM, Wednesday, 31 August 2005 Washington DC Convention Center -- 154A, Oral
Division of Analytical Chemistry |