Biological detection using surface-enhanced fluorescence on dye-labeled metal nanoparticles

ANYL 1

Jian Zhang, jian@cfs.umbi.umd.edu and Joseph R. Lakowicz. Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201
Fluorescence can be enhanced for a fluorophore localized near a surface of metal through coupling of a fluorophore with radiating metal plasmon. Such enhancement is dependent on particle size and shape, distance from fluorophore to the surface of metal, excitation and emission wavelengths of fluorophore, etc. When the metal particles are coupled each other, the enhancement effect becomes more obvious. This principle is utilized in biological detection including DNA/RNA, protein, and carbohydrate to improve the detection sensitivity.
 

General Papers
8:30 AM-11:35 AM, Sunday, 28 August 2005 Washington DC Convention Center -- 154A, Oral

Sci-Mix
8:00 PM-10:00 PM, Monday, 29 August 2005 Washington DC Convention Center -- Hall A, Sci-Mix

Division of Analytical Chemistry

The 230th ACS National Meeting, in Washington, DC, Aug 28-Sept 1, 2005