ANYL 146 |
| Investigations of the motion and chemistry of DNA in constrained environments provides important information on molecular organization and interactions. In this study, the self-diffusion coefficients of mononucleotides and oligonucleotides in solution were measured using 1H pulsed-field gradient NMR. Solutions of dextran in D2O (5%, 10%, and 20% by weight) act as a model of constrained environments with surface functionalities. The diffusion coefficients were measured for HDO, 2'-deoxyadenosine-5'-monophosphate, 2'deoxycytidine-5'-monophosphate, 2'-deoxyguanosine-5'monophosphate, and a number of olgionucleotides as a function of dextran solution concentration. Results are reported and compared to theoretical models based on the diffusion of spherical, elliptical, and cylindrical molecules. Based on these results, conclusions regarding the motion and interactions of nucleotides within these environments will be reported.
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General Papers
7:00 PM-9:00 PM, Sunday, 28 August 2005 Washington DC Convention Center -- Hall A, Poster
Sci-Mix
Division of Analytical Chemistry |