Optical spectroscopies for biological structure at interfaces

ANYL 294

Kimberly A. Briggman, kbriggma@nist.gov1, Neil Anderson1, John C. Stephenson1, and Lee J. Richter2. (1) Optical Technology Division, National Institute of Science and Technology, 100 Bureau Drive Stop 8443, Gaithersburg, MD 20899, (2) Surface and Microanalysis Division, National Institute of Science and Technology
In situ linear and novel nonlinear optical spectroscopies are being developed to probe biologically relevant, surface- and membrane-bound molecules. New interface-specific techniques based on infrared, Raman and vibrationally-resonant sum frequency generation (VR-SFG) spectroscopies are being used to study biological interfaces such as biological membrane mimics and incorporated membrane protein structures. These optical techniques can uniquely probe the structure of specific native functional groups of molecules without the need for molecular tagging. Moreover, the use of multiple techniques allows unambiguous determination of molecular structure and orientation of molecules at the interface. Presented results will include determination of the gel-fluid phase transition temperature of lipid layers in supported bilayer membranes, and the influence of membrane fluidity on the incorporation and structure of transmembrane polypeptides within the membrane.
 

Analytical and Biological Applications of Non-linear Optics
1:30 PM-5:10 PM, Monday, 29 August 2005 Washington DC Convention Center -- 155, Oral

Division of Analytical Chemistry

The 230th ACS National Meeting, in Washington, DC, Aug 28-Sept 1, 2005