Superparamagnetic nanoparticle supported enzymatic resolution of racemic carboxylates

ORGN 92

Hari Mohan Gardimalla, ghmreddy@siu.edu1, Deendayal Mandal, ddmandal@yahoo.com1, Philip Stevens, stevensphilip@yahoo.com1, Max Yen2, and Yong Gao, ygao@chem.siu.edu1. (1) Department of Chemistry and Biochemistry, Southern Illinois University, 224 Neckers Hall, Carbondale, IL 6901, (2) Materials Technology Center, Southern Illinois University, Carbondale, IL 62901
We report the use of superparamagnetic maghemite nanoparticles as a scaffold for recoverable biocatalysts. Candida rugosa lipase immobilized on maghemite nanoparticles demonstrated high stereoselectivity in kinetic resolution of racemic carboxylates, comparable to that of free lipase enzyme. The chemical immobilization of lipase on to the nanoparticles led to the decrease of the enzymatic activity but improved the long term stability over the parent free enzyme, allowing the supported enzyme to be repeatedly used for a series of chiral resolution reactions.

 

New Reactions and Methodology, Materials, Total Synthesis, Process R&D
8:00 PM-10:00 PM, Sunday, 28 August 2005 Washington DC Convention Center -- Hall A, Poster

Sci-Mix
8:00 PM-10:00 PM, Monday, 29 August 2005 Washington DC Convention Center -- Hall A, Sci-Mix

Division of Organic Chemistry

The 230th ACS National Meeting, in Washington, DC, Aug 28-Sept 1, 2005