Separation and characterization of zein from ground corn

AGFD 75

Fangyi Zhu, fyzhu@uiuc.edu, Aniket V. Kale, akale@uiuc.edu, and Munir Cheryan, mcheryan@uiuc.edu. Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 103 ABL, 1302 W. Pennsylvania Ave., Urbana, IL 61801
Zein is an alcohol-soluble protein derived from corn. Zein can be used for the manufacture of biodegradable materials and pharmaceutical applications. The whole ground corn was extracted with 70% ethanol at 50C for half an hour and filtered. The extract was passed through a Superdex Prep 75 chromatography column at 45C, with an alcoholic mobile phase. The fractions were characterized by HPLC and MALDI-TOF. Operating parameters (flowrate, sample size and composition of mobile phase) were optimized to improve the separation.
 

Sci-Mix
8:00 PM-10:00 PM, Monday, 14 March 2005 Convention Center -- Sails Pavilion, Sci-Mix

Division of Agricultural & Food Chemistry

The 229th ACS National Meeting, in San Diego, CA, March 13-17, 2005