Cytotoxic kaurene diterpenes from Laetia thamnia (Flacourtiaceae)

AGFD 68

Geneive E. Henry, henry@susqu.edu1, Jose C. Rosales2, Lynn S. Adams3, and Navindra P. Seeram, nseeram@mednet.ucla.edu3. (1) Department of Chemistry, Susquehanna University, 514 University Ave., Selinsgrove, PA 17870, (2) Chemistry Department, Susquehanna University, 514 University Ave., Selinsgrove, PA 17870, (3) UCLA Center for Human Nutrition, David Geffen School of Medicine, LA, CA 90095, Los Angeles, CA 90095, Los Angeles, CA 90095
The Laetia genus and several other plants in the Flacourtiaceae family reportedly contain clerodane or kolovane diterpenes, some of which are cytotoxic. However, there are no prior reports on phytochemical or biological studies of Laetia thamnia. Our phytochemical examination of the hexanes extract of L. thamnia leaves afforded four kaurene diterpenes, ent-3beta-hydroxykaur-16-ene, ent-kaur-16-en-3alpha,19-diol, ent-17-hydroxykaur-15-en-19-oic acid, and ent-kaur-16-en-19-oic acid. The isolates, the methyl ester of ent-kaur-16-en-19-oic acid and the acetate diester of ent-kaur-16-en-3alpha,19-diol were evaluated for cytotoxicity against human prostate (RWPE-1, 22Rv1, LNCAP), colon (HT 29, HCT 116, SW 480, SW 620), and breast (MCF 7) tumor cell lines. Compounds were tested at concentrations ranging from 6-50 mcg/mL, using a sensitive luminescent ATP cell viability assay. The antiproliferative activities against this panel of human tumor cell lines will be discussed. This is the first reported occurrence of kaurene diterpenes in the Laetia genus.

 

Sci-Mix
8:00 PM-10:00 PM, Monday, 14 March 2005 Convention Center -- Sails Pavilion, Sci-Mix

Division of Agricultural & Food Chemistry

The 229th ACS National Meeting, in San Diego, CA, March 13-17, 2005