AGFD 69 |
| It has been known that nobiletin in citrus fruits is a very important constituent with anticancer, antiviral and anti-inflammatory activities. Therefore, the demand for nobiletin in large quantity and high purity has increased dramatically. However, both the conventional normal phase silica gel chromatography and the C18-reverse phase separation methods cannot meet the demand of pure and gram scaled nobiletin in a timely manner. In the exploration of the content and the biological activities of polymethoxyflavones from sweet orange (Citrus Sinensis) peel, we have developed an efficient separation method of nobiletin. By employing this methodology, pure nobiletin in gram quantity was obtained in only one purification cycle. Thus, the orange peel extract (OPE) was loaded onto a silica gel flash column and the fractions eluted by a mixed solvent system of ethyl acetate and hexanes were collected. Upon concentration, the fraction mainly containing nobiletin and 5,6,7,4'-tetramethoxyflavone was loaded onto a Regis chiral column. Then nobiletin and 5,6,7,4'-tetramethoxyflavone were eluted with ethanol and hexanes. Detailed experiment procedure, comparison of efficiency with conventional normal and reverse phase, and possible explanation will be presented. |
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Sci-Mix
8:00 PM-10:00 PM, Monday, 14 March 2005 Convention Center -- Sails Pavilion, Sci-Mix
Division of Agricultural & Food Chemistry |