Application of digital filters to enhance conformational change in protein systems

COMP 84

Adrian P. Wiley and Jonathan W Essex, jwe1@soton.ac.uk. School of Chemistry, University of Southampton, Highfield, Southampton, SO17 1BJ, United Kingdom
Motions in biological molecules can occur on timescales beyond those accessible to molecular dynamics simulations, and algorithms that enhance conformational sampling are therefore of considerable interest. Reversible Digitally Filtered Molecular Dynamics (RDFMD) is able to enhance sampling by amplifying the low frequency motions as a simulation evolves. In this presentation, methods to optimise the performance of RDFMD are described, and applications of this approach to enhancing the conformational motion in E. coli dihydrofolate reductase, T4 lysozyme, and HIV-1 protease, are described.