BIOT 257 |
| One of the major challenges in proteomics is the identification of trace proteins in complex protein mixture. Since displacement chromatography has the ability to carry out simultaneous concentration and high resolution separations, it is uniquely well suited for proteomic applications. In this work, high affinity displacers for ion exchange systems were identified using parallel batch experiments to determine the concentration of displacer required to displace 50% of the adsorbed biomolecules (DC-50). Using this approach, a large number of high affinity displacers were identified that could displace the adsorbed biomolecules at relatively low concentrations. In addition, the DC-50 data were employed in concert with molecular structural information of the displacers to produce predictive quantitative structure-efficacy relationship (QSER) models based on a support vector machine (SVM) regression approach. Several of the high affinity displacers were then employed for concentration and purification of peptides from complex protein digests and the ability of this technique to identify trace components was demonstrated. Furthermore, the ability to use these high affinity displacers at relatively low displacer concentrations in order to produce wider displaced zones, higher purities and improved trace component detection was demonstrated. |
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Poster Session
5:30 PM-7:30 PM, Wednesday, 16 March 2005 Convention Center -- Sails Pavilion, Poster
Division of Biochemical Technology |