ANYL 71 |
| Xinhui Lou and Lin He. Department of Chemistry, North Carolina State University, 2620 Yarbrough Dr, Raleigh, NC 27695 |
| The detection of specific nucleotide sequences is critical to the diagnosis of genetic and pathogenic disease. Herein we present a new method for the detection and quantification of target DNA hybridized with probe DNA on a solid surface by monitoring the growth of specific polymeric molecules. Initiators are immobilized at the end of target DNA. These initiators are brought to the array surface through hybridization, but are absent where non-complementary targets are spotted. Subsequent atom transfer radical polymerization (ATRP) introduces significant mass changes that can be detected using surface plasmon resonance, Infrared spectroscopy, X-ray photoelectron spectroscopy and ellipsometry. An unlimited amplification is possible by regulating the final molecular weight and/or the side chain structure of the polymer formed, which may provide sufficient sensitivity to enable single nucleotide polymorphism (SNP) detection in genomic DNA of high complexity without a PCR amplification. |
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General Papers
7:00 PM-9:00 PM, Sunday, August 22, 2004 Pennsylvania Convention Center -- Hall D, Poster
Division of Analytical Chemistry |