COLL 368 |
| Steven G. Boxer, Department of Chemistry, Department of Chemistry, Stanford University, Stanford University, Stanford, CA 94305-5080 |
| It has proven to be quite difficult to incorporate membrane proteins into supported bilayers. We have now solved this problem by preparing lipids with DNA molecules as head groups. When displayed on supported bilayers, they can be used to tether vesicles of arbitrary size and composition that display the antisense DNA. This can be used to create arrays of tethered vesicles containing membrane proteins on patterned supported bilayers. Tethered vesicles are laterally mobile in the plane of the supported bilayer, so individual vesicle-vesicle interactions, mediated by different components on the vesicle surface or in solution, can be observed directly. Switchable barriers have been developed to trigger interactions. A microfluidic separation system has been developed to create purer populations of vesicles so that multivalent interactions can be probed quantitatively. Progress towards the analysis of membrane lateral composition using multi-isotope imaging mass spectrometry will also be described. |
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Surface and Colloid Chemistry Award Symposium Honoring Joseph Zasadzinski
8:10 AM-12:10 PM, Wednesday, March 31, 2004 Marriott -- Orange County 3, Oral
Division of Colloid and Surface Chemistry |