COLL 284 |
| Chengzhi Cai, Jianhua Gu, Chi Ming Yam, Sha Li, and Guotin Qin. Department of Chemistry, University of Houston, 136 Fleming Building, Houston, TX 77204-5003 |
| The ability to control the location of individual bio-molecules on bio-compatible surfaces will open new possibilities for biological research at nanoscale. Towards this goal, we have developed a new, robust system based on oligo(ethylene glycol) (OEG) monolayers grown by hydrosilylation (forming Si-C bonds) of OEG-terminated alkenes on hydrogen-terminated silicon surfaces. The results of ellipsometry, x-ray photoelectron spectroscopy, fluorescent imaging, and atomic force microscopy (AFM) studies showed that the OEG films prepared under optimized conditions strongly resisted the non-specific adsorption of a variety of proteins. We demonstrated that these atomically flat monolayers can be patterned by a biased AFM tip. The patterned areas bind a variety of proteins. In this way, we have prepared arrays of avidin spots. The diameters of the protein spots with an adjustable spacing are currently as small as 20 nm. |
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Nanoscience and Nanotechnology
6:00 PM-8:00 PM, Monday, March 29, 2004 Disneyland -- North Exhibit Hall, Poster
Division of Colloid and Surface Chemistry |