COLL 213 |
| Shelia Maness1, Lee J. Richter2, and Kimberly A. Briggman1. (1) Optical Technology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, (2) Surface and Microanalysis Division, National Institute of Science and Technology |
| Novel nonlinear optical spectroscopies are rapidly advancing techniques for determining the structure and orientation of biological molecules at surfaces. Broadband vibrationally resonant sum frequency generation (VR-SFG) spectroscopy is uniquely interface specific, has monolayer sensitivity, and is well suited for biological studies due to short spectral acquisition times. A new variation of VR-SFG, doubly-resonant sum frequency generation (DR-SFG), exploits both the natural vibrational and electronic resonances of a surface-bound molecule, thus making the technique much more sensitive to lower surface coverages, appropriate for physiological concentrations at biological interfaces. This talk will present the orientational analysis of aromatic amino acid residues (Trp, Tyr, and Phe) self assembled on a gold surface through a cysteine thiolate bond. Both VR-SFG and DR-SFG data will be presented and analyzed for absolute amino acid residue orientation. |
|
Posters: Fundamental Research in Surface and Colloid Chemistry
6:00 PM-8:00 PM, Monday, March 29, 2004 Disneyland -- North Exhibit Hall, Poster
Division of Colloid and Surface Chemistry |