I&EC 21 |
| Eva Oberdörster, Department of Biology, Department of Biology, Southern Methodist University, 6501 Airline Road, Dallas, TX 75275-0376 |
| The goal of this study was to evaluate whether the nC60 fullerenes induce oxidative stress in two standard aquatic test species. Neonatal daphnids were individually exposed in 40 mL of media augmented with fullerenes in the range of 5 ppb to 2 ppm with a media change every 2-3 days for three weeks (EPA standard test protocol). The 48 hour LC50 for daphnia was 460 ppb as calculated in two separate trials. At 500 ppb and above, the surviving daphnids were delayed in molting and reproduction. Juvenile largemouth bass were exposed to either 0.5 or 1 ppm fullerenes for 48 hours. For both daphnia and bass, lipid peroxidation and protein oxidation were analyzed and compared to a positive control of 100 mM hydrogen peroxide exposure. In addition, largemouth bass livers from the 1 ppm dose were analyzed by subtractive hybridization for changes in gene expression due to fullerene exposure. |
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Nanotechnology and the Environment
12:15 PM-2:30 PM, Sunday, March 28, 2004 Marriott -- Grand Ballroom F, Oral
Division of Industrial and Engineering Chemistry |