COLL 206 |
| Patrick Lim Soo1, Stanislav N. Sidorov2, Jeannie Mui3, Lyudmila M. Bronstein4, Hojatollah Vali3, Adi Eisenberg1, and Dusica Maysinger5. (1) Department of Chemistry, McGill University, 801 Sherbrooke Street West, Lab 436, Montreal, QC H3A 2K6, Canada, (2) Nesmeyanov Institute of Organoelement Compounds RAS, 28 Vavilov str, Moscow, 119991, Russia, (3) Department of Anatomy and Cell Biology, McGill University, 3640 University St., Montreal, QC H3A 2B2, Canada, (4) Department of Chemistry, Indiana University, 800 E Kirkwood Av, Bloomington, IN 47405, (5) Department of Pharmacology and Therapeutics, McGill University, 3655 Promenade Sir William Osler, Montreal, QC H3G 1Y6, Canada |
| Block copolymer micelles have potential applications in the medical, cosmetic and environmental fields. Their subcellular fate in vitro and in vivo can be assessed using a heavy metal label. Gold incorporated poly (4-vinyl pyridine)-block-poly (ethylene oxide) micelles were prepared ranging in size from 5-10 nm to investigate the cellular internalization into different cell lines. The micelles were characterized using UV-vis, dynamic light scattering (DLS), transmission electron microscopy (TEM) and energy dispersive spectrometry (EDS). The gold incorporated micelles were internalized into the human epithelial (HEK 293) and human lung carcinoma (A549) cells for time periods up to 24 hours. The internalization of gold incorporated micelles in two different cell types and their subcellular distribution are shown using TEM. In addition, EDS provides evidence of the gold particles inside of the cellular organelles (i.e. endosomes or lysozomes). The gold incorporated P4VP-b-PEO micelles are useful tools for the visualization and subcellular distribution of micelles at a high level of resolution. |
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Posters: Fundamental Research in Surface and Colloid Chemistry
6:00 PM-8:00 PM, Monday, March 29, 2004 Disneyland -- North Exhibit Hall, Poster
Division of Colloid and Surface Chemistry |