COLL 203 |
| Guy G. Ting II, Nicole M. Kunze, and Kimberly A. O. Pacheco. Department of Chemistry and Biochemistry, University of Northern Colorado, Ross Hall, Campus Box 98, Greeley, CO 80639 |
| Thin films were constructed using N-(6-aminohexyl)aminopropyltrimethoxysilane (NAHAPTMS) on hydroxylated mica surfaces and were characterized using fourier transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), and scanning electron microscopy (SEM). Once the thin film is in place, it can be modified by attaching a receptor molecule to the terminal amino group on the silane. This project takes advantage of the specificity between antibodies and their analytes for proof-of-concept studies. Films using NAHAPTMS labeled with 5-carboxyfluorescein were formed on mica surfaces. The ratio of silane versus fluorescein-labeled silane making up the thin film was varied to give the optimum coverage and then characterized by FT-IR, AFM, SEM, confocal microscopy, and quenching experiments using antibodies specific for fluorescein. |
|
Posters: Fundamental Research in Surface and Colloid Chemistry
6:00 PM-8:00 PM, Monday, March 29, 2004 Disneyland -- North Exhibit Hall, Poster
Division of Colloid and Surface Chemistry |