COLL 287 |
| Muditha D. Senarath-Yapa, S. Scott Saavedra, Craig A. Aspinwall, and David L. Roberts. Department of Chemistry, University of Arizona, 1306E University Blvd., Tucson, AZ 85721-0041 |
| We are investigating the use of poly(lipid)-coated, dye doped silica nanoparticles, bearing ligands to receptor analytes, as biomarkers and biosensors. These structures have several advantages as biomarker/sensor materials, such as: a) the lipid monomers are crosslinked to create a highly stable particle coating, b) the phosphorylcholine headgroups of the lipids minimize nonspecific adsorption of proteins, c) the nanoparticle contains multiple dye molecules, which generates an easily detectable fluorescence signal upon receptor/ligand binding. Furthermore, dye molecules entrapped within a silica nanoparticle are resistant to photobleaching. Silica nanoparticles, ca. 65 nm in diameter and doped with Ru(bpy)32+, were synthesized using an inverse micelle method. A lipid bilayer coating was deposited by vesicle fusion, followed by lipid polymerization. A variety of techniques including TEM, light scattering, and fluorescence spectroscopy and imaging have been used to characterize these structures. Applications of their use in biosensing and bioimaging will be discussed. |
|
Nanoscience and Nanotechnology
6:00 PM-8:00 PM, Monday, March 29, 2004 Disneyland -- North Exhibit Hall, Poster
Division of Colloid and Surface Chemistry |