COLL 490 |
| Pinaki R. Majhi, Department of Pharmaceutical Chemistry, School of Pharmacy, University of California San Francisco, San Francisco, CA 94143 and Alfred Blume, Institute of Physical Chemistry, Martin-Luther-University Halle-Wittenberg, Muehlpforte 1, D-06108 Halle/Saale, Germany. |
| We studied the surfactants and surfactant-phospholipid mixed systems using isothermal titration calorimetry (ITC) and differential titration calorimetry (DSC). The critical micellar concentration (cmc) of surfactant and the partitioning of surfactant into lipid bilayers, followed by solubilization of vesicles, exhibit a characteristic temperature dependence, which is due to hydrophobic interactions. ITC data were used for predicting possible changes in aggregate structure as a function of temperature and composition. The ITC study on surfactant systems shows the possibility of determining cmc of surfactants using DSC, which has been verified both theoretically and experimentally. From the ITC study on a particular lipid-surfactant system we predicted the temperature for the micelle-vesicle transition, which was verified by DSC and turbidity measurements. This study offers new possibilities in biological membranes, namely, a solubilization procedure of detergent saturated membranes by cooling or reconstitution of proteins into bilayers by warming. |
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Symposium in Memory of Arthur W. Adamson
8:30 AM-11:50 AM, Thursday, April 1, 2004 Marriott -- Orange County 4, Oral
Division of Colloid and Surface Chemistry |