COLL 234 |
| David Dunmire and Angela Hight Walker. Optical Technology Division - Physics laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Stop 8441, Gaithersburg, MD 20899 |
| Surface enhanced Raman spectroscopy (SERS) is employed to study biotin and the biotin-avidin complex in a microfluidic device. The microfluidic device enables rapid, controlled modifications to the chemical environment to induce structural changes in the biomolecules. Gold and silver colloids are used as SERS substrates and are produced either in situ or injected already synthesized. The enhanced Raman intensity, derived from the nanoparticles, enables the study of solution-phase biotin. Biotin has a low solubility (0.2 mg/ml) in aqueous media at physiological conditions and is a weak Raman scatterer. This fact limited previous research to monitoring the avidin structural changes in the complex upon binding. Here, we monitor changes of the biotin vibrational spectra to deduce new structural insight into the complex. |
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Posters: Fundamental Research in Surface and Colloid Chemistry
6:00 PM-8:00 PM, Monday, March 29, 2004 Disneyland -- North Exhibit Hall, Poster
Division of Colloid and Surface Chemistry |