COLL 185 |
| Brent Matteson, Margo Rockwell, Daniel Roston, and John LaGraff. Chemistry, Hamilton College, 198 College Hill Road, Clinton, NY 13323 |
| Patterning of biomolecules onto various substrates using microcontact printing (mcp) has potential application in the fabrication of biological devices such as biosensors, microarrays, and protein chips or as substrates for directed cell growth. Effectiveness of these devices will depend strongly on maintaining the functionality of the biomolecules once they are attached to a surface. In this research, antibodies (i.e., immunoglobulins) were attached to a glass substrate by either mcp or direct physisorption from solution, and then incubated in solutions of fluorescently labeled secondary antibodies (the antigen). Relative concentrations of bound antigens, and hence the binding efficiency of the antibodies, were determined using epi-fluorescent microscopy. Results indicated that a greater concentration of antigens was bound to mcp antibodies than to those antibodies physisorbed on glass directly from solution. Binding efficiency of antibodies was also measured as a function of temperature, protein solution concentration, and stamp drying time. |
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Posters: Fundamental Research in Surface and Colloid Chemistry
6:00 PM-8:00 PM, Monday, March 29, 2004 Disneyland -- North Exhibit Hall, Poster
Division of Colloid and Surface Chemistry |