COLL 340 |
| Mu-Ping Nieh1, V. A. Raghunathan2, Mukudaan Chakrapani1, Thad A. Harroun1, and John Katsaras1. (1) neutron program for materials research (NPMR), NRC, SIMS, Bldg 459, Chalk River Lab, Chalk River, ON K0J-1J0, Canada, (2) Raman Research Institute, Raman Research Institute, Bangalore, 560 080, India |
| “Bicelles” composed of long-chain (14-C dimyristoyl phosphatidylcholine) and short-chain (6-C dihexanoyl phosphatidylcholine) phospholipid mixtures are magnetically alignable in an aqueous environment and have been used as substrates to study the structure of associated proteins or biomolecules. Using small-angle neutron scattering (SANS) and optical microscopy we observe, with increasing temperature, an isotropic-to-nematic and a nematic-to-smectic transition. We have also investigated the effect of surface charge density on bilayer alignment. Neutron diffraction results show that a weak shear can align the system equally or better than the system doped with paramagnetic ions and aligned in a magnetic field. This method of alignment eliminates both the use of strong magnetic fields and paramagnetic ions, which may bind with the proteins, potentially altering their functionality. |
|
Bio-Colloids
2:00 PM-4:40 PM, Tuesday, March 30, 2004 Marriott -- Orange County 3, Oral
Division of Colloid and Surface Chemistry |