COLL 43 |
| Lee J. Richter1, Clayton S-C. Yang2, Kimberly A. Briggman2, and John C. Stephenson2. (1) Surface and Microanalysis Science Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, (2) Optical Technology Division, National Institute of Standards and Technology, 100 Bureau Dr MS 8443, Gaithersburg, MD 20899 |
| Hydrated supported phospholipid (PL) structures (Langmuir-Blodgett films, supported bilayers, etc.) have been widely studied as model systems for biological membranes. Hybrid bilayer membranes (HBM: PL monolayer on a self-assembled monolayer (SAM) support) are of particular interest, as they allow independent control of each of the leaflets of the ‘bilayer’ and facilitate electrochemical interrogation. We have used vibrationally-resonant sum frequency generation (VR-SFG) to characterize the formation and behavior of HBMs. Kinetic studies of the formation of the SAM and PL layers establish the coexistence of multiple phases and their influence on adsorption dynamics. The unique sensitivity of VR-SFG to alkane chain order allows detailed study of the gel-fluid phase transition temperature Tm of the supported PL. We demonstrate that the nature of the SAM support allows control over Tm. The HBM supports have been used to vector orient membrane peptides, facilitating spectroscopic studies of their structure and kinetics. |
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Vibrational Analyses of Dry and Wet Surfaces
2:00 PM-5:45 PM, Sunday, March 28, 2004 Marriott -- Grand Ballroom H, Oral
Division of Colloid and Surface Chemistry |