COLL 489 |
| Alexander Yaroslavov1, Anna A. Efimova2, Anna A. Rakhnyanskaya2, Ekaterina G. Yaroslavova2, and Nickolay S. Melik-Nubarov2. (1) Department of Polymer Sciences, School of Chemistry, M.V. Lomonosov Moscow State University, Leninskie Gory, Moscow, 119899, Russia, (2) School of Chemistry, Moscow State University, Leninskie Gory, Moscow, 119899, Russia |
| Complexation of negative (proteo)liposomes with synthetic polycations was studied. The interaction could be accompanied by lateral lipid and protein segregation, highly accelerated transmembrane migration of lipid molecules, incorporation of adsorbed polycations into liposomal membrane, increase in membrane permeability towards simple ions and non-charged molecules as well as aggregation and disruption of liposomes. Electrostatic binding of polycations had no effect on functional activity of enzymes incorporated into the membrane. Stability of liposome-polycation complexes in water salt solutions and in the presence of competing polyions could be modulated by changing the content of negative lipid in and the phase state of the membrane, size of liposomes as well as polycation composition. The obtained results and conclusions we made might be useful to interpret biological effects of polyelectrolytes and multicharged polymeric constructs. |
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Symposium in Memory of Arthur W. Adamson
8:30 AM-11:50 AM, Thursday, April 1, 2004 Marriott -- Orange County 4, Oral
Division of Colloid and Surface Chemistry |