COLL 542 |
| Buddy D. Ratner1, Xuanhong Cheng1, Yanbing Wang2, Yael Hanein2, and Karl F. Böhringer2. (1) Department of Bioengineering, University of Washington, Bagley Hall Room 484, Box 351720, Seattle, WA 98195, (2) Department of Electrical Engineering, University of Washington, 234 EE/CSE Building, Box 352500, Seattle, WA 98195 |
| A new method for protein and cell patterning based on addressable microheaters and a poly(N-isopropyl acrylamide)(pNIPAM) thermoresponsive coating is reported here. This thermoresponsive coating is created by radio frequency NIPAM plasma deposition and is denoted as plasma polymerized NIPAM (ppNIPAM). Films of ppNIPAM with a good retention of monomer side-chain functionality are produced using low power continuous plasma deposition. Protein adsorption, cell adhesion and cell detachment tests indicate that the surface switches between adhesive and non-adhesive behaviors as a function of temperature. The use of a photolithographically fabricated microheater array allows the ppNIPAM transition to occur spatially under the control of individual heaters. This localized change in the surface adhesive behavior is used to direct site-specific cell attachment. Patterned adhesion of two types of cells has been visualized on the array through fluorescent markers. Applications for diagnostic devices, cell-based sensors, tissue engineering and cell transfection are envisioned. |
|
“Smart” Polymers on Surfaces and Colloids
2:00 PM-4:50 PM, Thursday, April 1, 2004 Marriott -- Grand Ballroom J, Oral
Division of Colloid and Surface Chemistry |