COLL 233 |
| Jiangyue Zhang, Department of Chemistry 016-00, Temple University, 13th and Norris Streets, Beury Hall, Philadelphia, PA 19122, George R. Baran, Department of Mechanical Engineering, Temple University, College of Engineering, 1947 N. 12th Street, Philadelphia, PA 19122, and Stephanie L. Wunder, Department of Chemistry, 016-00, Temple University, 13th. & Norris St., Philadelphia, PA 19122. |
| Supported lipid membranes are used in biosensor devices and in preparation of biocompatible surfaces. For some applications, solid supports that are spherical rather than planar are needed. In these cases, Langmuir-Blodgett deposition of the bilayer cannot be used. In the current investigation, bilayers were formed by covalent attachment of octadecyltrichlorosilane (OTS) to 70 and 100nm SiO2 beads, followed by fusion of DPPC vesicles onto the OTS surface. The OTS formed a close packed structure, as characterized by thermogravimetric (TGA) analysis and Raman spectroscopy. When sonicated with DPPC vesicles, lipid attachment occurred and could be monitored after centrifugation using TGA. TGA weight loss of the lipid was a distinct step in the TGA plot, and corresponded to successive bilayer additions. Use of OTS/deuterated DPPC and deuterated OTS/DPPC permitted separate monitoring of the melt transition of the attached OTS and unattached lipid. Supported by NIH DE09530. |
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Posters: Fundamental Research in Surface and Colloid Chemistry
6:00 PM-8:00 PM, Monday, March 29, 2004 Disneyland -- North Exhibit Hall, Poster
Division of Colloid and Surface Chemistry |