COLL 85 |
| Gerald W. Feigenson, Frederick Heberle, and David Stringer. Field of Biophysics, Cornell University, 201 Biotechnology Building, Ithaca, NY 14853 |
| Determination of all of the phase boundaries in 3-component lipid bilayer mixtures is a difficult problem. Fluorescence resonance energy transfer (FRET) experiments, by use of probes having appropriate partition behavior between coexisting phases, can be sensitive to the phase separations. When phases coexist, FRET is enhanced if both donor and acceptor partition into the same phase. In this case, sensitivity is greatest near the phase boundary where there is only a small amount of the favored phase. A different FRET experiment makes use of donor and acceptor separating from each other when lipid phases coexist, yielding reduced FRET. We have achieved good definition of phase boundaries in sphingomyelin/DOPC/cholesterol bilayer mixtures and in DSPC/DOPC/cholesterol mixtures that contain four fluorescent dyes: two different donor/acceptor pairs, namely dehydroergosterol/perylene and 18:2,18:2-DiO/18:2,18:2-DiI. |
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Bio-Colloids
8:30 AM-11:30 AM, Monday, March 29, 2004 Marriott -- Grand Ballroom K, Oral
Division of Colloid and Surface Chemistry |