I&EC 235 |
| Feimeng Zhou1, Jianxiu Wang2, and Hui Wang2. (1) Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, CA 90032, (2) Department of Chemistry, Graduate School, Chinese Academy of Sciences, 19A Yu Quan Road, Beijing, 100039, China |
| Metal detoxification is a cellular or physiological process that ameliorates, reduces, or eliminates toxicity given rise by metals. Metallothioneins (MTs), a class of cysteine-rich, low molecular weight proteins, and their related oligopeptides, are purported to play important roles in environmental toxicology by sequestering and transferring (eliminating) heavy metals. At issues are whether the metal transfer is modulated by cellular redox reactions or mediated by certain intermediaries that may assist the metal transfer between MTs and a substrate. To understand the metal transfer process, methods are needed to quantify the cysteine residues per MT molecule immobilized onto an electrode surface. We have developed a nanoparticle-amplified voltammetric detection scheme for the analysis of surface-confined DNA and peptides at ultratrace levels. In this presentation, we will describe the application of such a scheme to the sensing and quantification of free cysteine residues of MTs and/or peptides that have undergone metal transfer reactions. |
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Nanotechnology and the Environment
1:30 PM-4:35 PM, Wednesday, March 31, 2004 Marriott -- Grand Ballroom F, Oral
Division of Industrial and Engineering Chemistry |