COLL 315 |
| Abhay S. Vaze1, Michael Parizo2, and James F. Rusling1. (1) Department of Chemistry, University of Connecticut, 55 North Eagleville Road, Storrs, CT 06269, (2) Pfizer Pharmaceuticals, Eastern Point Rd, Groton, CT |
| Redox proteins attached to surfaces designed for biocatalysis hold promise for future clean synthetic routes. It would be advantageous for these biocatalysts to operate in low-toxicity fluids with high capacity to dissolve reactants. Here we report that crosslinked films of myoglobin (Mb) and poly(L-lysine) (PLL) attached layer-by-layer to oxidized carbon cloth cathodes gave greatly improved turnover rates for epoxidation of styrene in microemulsions. Up to 40-fold larger turnover rates were found in bicontinuous microemulsions compared to o/w microemulsions and micelles. Larger turnover rates are correlated with up to 10-fold faster diffusion of solutes in the oil phases of the bicontinuous fluids. Better reactant availability in the bicontinuous fluids, possibly featuring increased interphase solute exchange mediated by high cosurfactant content, may aid in improving turnover rates. Mb/PLL films crosslinked at each layer of formation were the most stable in microemuslions and had best turnover rates compared to crosslinked/uncrosslinked Mb/poly(styrene sulfonate) films. |
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Symposium in Memory of Arthur W. Adamson
8:30 AM-11:10 AM, Tuesday, March 30, 2004 Marriott -- Orange County 4, Oral
Division of Colloid and Surface Chemistry |