COLL 242 |
| Joonyeong Kim, Telly S. Koffas, and G. A Somorjai. Department of Chemistry, University of California and Lawrence Berkeley National Laboratory, Berkeley, CA 94720 |
| The controlled immobilization of proteins is crucial in the field of biotechnology where well-ordered protein layers may lead to a new generation of reactor beds in catalysis and biosensor applications, as well as in disease diagnostics. Previous studies using various physical and analytical tools have shown that protein adsorption is a complex phenomenon involving a series of dynamic steps from initial contact at the interface to the final structural rearrangements on the solid substrate. The choice of solid substrate and physiological adsorption conditions (e.g., temperature, pH, and concentration of bulk protein solutions) are known to significantly affect the overall adsorption kinetics, the amount of adsorbed proteins, and the degree of denaturation. The goal of this project is to investigate the effect of adsorption conditions on the structure of adsorbed proteins using infrared-visible sum frequency generation (SFG) vibrational spectroscopy, fluorescence microscopy (FM), and atomic force microscopy (AFM). To this end, the ordering and orientation of immunoglobulin G (IgG) adsorbed on hydrophilic and hydrophobic surfaces is investigated as a function of bulk protein concentration and pH. In addition, the structural information obtained from SFG is compared to the data from AFM and FM to determine the extent of global and local ordering in adsorbed proteins. |
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Posters: Fundamental Research in Surface and Colloid Chemistry
6:00 PM-8:00 PM, Monday, March 29, 2004 Disneyland -- North Exhibit Hall, Poster
Division of Colloid and Surface Chemistry |