COLL 482 |
| Etsuo Kokufuta, Institute of Applied Biochemistry, Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8565, Japan |
| Complexation of proteins with polymers in aqueous media is of great importance for various fields of pure and applied science. Of particular interest would be studies on the mechanism of complexation as well as on the molecular characteristic of resulting complexes. From this point of view, we focused on protein complexes of water-soluble polymers with charges [e.g., Langmuir 12, 6295 (1996); 16, 3133 (2000)] and without charges [e.g., Macromolecules 26, 6688 (1993); Langmuir, 15, 940 (1999); Biomacromolecules, 4, 728 (2003)]. From light scattering experiments, it has become apparent that either intra- or inter-molecular complex particles with diameters in the range of tens to hundreds of nanometers are formed dependent on factors such as a mixing ratio of polymer and protein. The molecular weight and the number of bound proteins per polymer were then determinable, allowing us to discuss the complexation mechanism at the molecular level. |
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Symposium in Memory of Arthur W. Adamson
8:30 AM-11:50 AM, Thursday, April 1, 2004 Marriott -- Orange County 4, Oral
Division of Colloid and Surface Chemistry |