COLL 483 |
| Udayan Mohanty, Department of Chemistry, Department of Chemistry, Boston College, Chestut Hill, Newton, MA 02467 |
| We have explicitly solved the longstanding problem of a quantitative predictive model that describes the electrophoretic mobility of curved DNA fragments in polyacrylamide gels. The curvature in the DNA is due to short stretches of adenines (A-tracts) which were repeated in phase with the helical repeat. The model takes into account in an approximate way polyelectrolyte effects such as condensed and screened counterions, coulombic end effects, salt concentration, pH of the buffer, screening of the hydrodynamic interactions, flexibility of the molecule, conformation fluctuations, and the characteristics of the interactions of the gel with the curved DNA. The predictions are in excellent agreement with experimental data of Crothers and coworkers. A generalized of our model leads to a description of the dynamics of phased A-tracts, where the A-tracts containing sequences are located at different phasing with respect to a bend of known magnitude. |
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Symposium in Memory of Arthur W. Adamson
8:30 AM-11:50 AM, Thursday, April 1, 2004 Marriott -- Orange County 4, Oral
Division of Colloid and Surface Chemistry |