COLL 538 |
| Sandeep S. Karajanagi, Dae-Yun Kim, Ravindra S. Kane, and Jonathan S. Dordick. Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, 110, 8th street, Troy, NY 12180 |
| Incorporating enzymes on carbon nanotubes has opened up opportunities for making novel biomaterials with applications ranging from biosensors and biomedical devices to self-healing and self-cleaning surfaces. Here we report the non-covalent and covalent functionalization of Single-Walled Carbon Nanotubes (SWNTs) and Multi-Walled Carbon Nanotubes (MWNTs) with enzymes for generating biocatalytic nanomaterials. Soybean Peroxidase (SBP) and Chymotrypsin (CT) were immobilized onto SWNTs using adsorption. SBP retained 28% of its native activity upon immobilization while CT lost almost all of its activity. Secondary structure elucidation using FT-IR spectroscopy revealed that SBP retains near native secondary structure upon immobilization on SWNTs, whereas CT loses most of its secondary structure. The enzymes were also covalently coupled to oxidized MWNTs and N-doped MWNTs by reacting the amine functionalities on the proteins with carboxylic acid functionalities on MWNTs with a carbodiimide derivative and N-hydroxysuccinimide. The resulting nanocomposites were incorporated into polymers, which were highly active and stable. Incorporating enzyme-nanotube composites into polymers provides a direct route to the design of nonfouling surfaces for potential use in biomedical devices and coatings. |
|
Nanoscience and Nanotechnology
2:00 PM-5:15 PM, Thursday, April 1, 2004 Marriott -- Orange County 5, Oral
Division of Colloid and Surface Chemistry |