COLL 334 |
| Alexey A. Vertegel1, Richard W. Siegel1, and Jonathan S. Dordick2. (1) Materials Science and Engineering and Rensselaer Nanotechnology Center, Rensselaer Polytechnic Institute, Troy, NY 12180, (2) Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, 110 8th St, Troy, NY 12180 |
| In spite of the fact that much work has been done on protein – nanoparticle conjugation, no systematic study of the effect of nanoparticle size on the structure and activity of adsorbed proteins has been performed. Here, we study conformation and activity of lysozyme adsorbed on colloidal silica nanoparticles with various sizes. Both adsorption patterns and protein properties are found to be strongly dependent on the size of the nanoparticles. Adsorption behavior changes from the formation of “stoichiometric” aggregates with a discrete amount of protein molecules adsorbed to each SiO2 particle (4 nm silica) to monolayer adsorption (20nm silica) and to multilayer adsorption (100 nm silica). Structural changes upon adsorption measured as a loss of the protein &alpha-helix content were found to be more pronounced in the case of bigger nanoparticles. In good agreement with the previous observation, activity of the adsorbed protein decreased with the increase of the particle size. |
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Bio-Colloids
2:00 PM-4:40 PM, Tuesday, March 30, 2004 Marriott -- Orange County 3, Oral
Division of Colloid and Surface Chemistry |