Displacement chromatography of complex protein mixtures for proteomic applications

BIOT 282

Jia Liu and Steven M. Cramer. Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180
One of the major challenges in proteomics is the identification of trace proteins and the limited dynamic range. Since displacement chromatography has the ability to carry out simultaneous concentration and high-resolution separations it is uniquely well suited for proteomic applications. In this poster, the utility of ion exchange displacement chromatography was examined for high-resolution separations of complex protein mixtures. High affinity low molecular weight displacers were employed in concert with nonporous small particle diameter ion-exchangers to develop these efficient separations. The high affinity displacers enabled us to operate at relatively low displacer concentrations, resulting in wider displaced protein zones, higher yields and larger dynamic ranges of proteins that could be separated. The use of nonporous resins resulted in extremely sharp boundaries between the displaced protein zones, further increasing the dynamic range of detectable proteins. Through the use of standard chromatographic models, the effect of the shock layer in these systems was studied and the separations were optimized. The results in this poster demonstrate that the combination of high affinity displacers and non-porous ion-exchange resins have significant potential for increasing the dynamic range of proteins that can be identified for proteomic applications.
 

Poster Session
5:30 PM-7:30 PM, Wednesday, March 31, 2004 Anaheim Convention Center -- Hall A, Poster

Division of Biochemical Technology

The 227th ACS National Meeting, Anaheim, CA, March 28-April 1, 2004