Multiplexed analyses of serum antibodies to Bacillus anthracis toxins from cases with cutaneous anthrax in the former Soviet Republic of Kazakhstan


Deborah L. Sammons1, Jerome P. Smith1, Raymond E. Biagini1, John E. Snawder1, Cynthia A. F. Striley1, Shirley K. Robertson1, Barbara A. MacKenzie1, Patty P. Wilkins2, and Conrad P. Quinn2. (1) Biomonitoring and Health Assessment Branch, CDC/NIOSH, 4676 Columbia Parkway, Cincinnati, OH 45226, (2) Microbial Pathogenesis and Immune Response Laboratory, CDC/NCID, 1600 Clifton RD, Atlanta, GA 30333
Bacillus anthracis (Ba) exotoxins, lethal factor (LF) and protective antigen (PA) are immunogenic, and exposure may be evaluated by measurement of specific antibodies by multiplexed fluorescence covalent microbead immunoassay (FCMIA). Using sera from individuals with "confirmed" cutaneous cases of (N=7) of anthrax from the former Soviet Republic of Kazakhstan (Kaz) and 8 donor controls we found 47.9 37.4 and 19.0 16.3, 1.00 0.98 and 0.14 0.12 g/ml anti-LF IgG and anti-PA IgG, respectively (Kaz and control; mean Standard Deviation [SD]; both P<0.001). For anti-LF IgM and anti-PA IgM, we found 1107 999 and 265 174, 33.5 29.7 and 9.6 6.0 (Kaz and control; standard sera equivalent dilution SD; P<0.001), respectively. These data indicate that cutaneous anthrax infection can induce significant levels of anti-LF and anti-PA IgG and IgM which can be measured by multiplexed FCMIA.


General Posters
1:00 PM-3:00 PM, Monday, September 8, 2003 Javits Convention Center -- Hall 1B/1C, Poster

Division of Agrochemicals
The 226th ACS National Meeting, New York, NY, September 7-11, 2003