Recent advances in the fluorescence detection of carbohydrates


Zeev Rosenzweig, Yongfen Chen, and Gabriela Blagoi. Department of Chemistry, University of New Orleans, NA, New Orleans, LA 70148
The presentation focuses on new advances in the development of luminescence nanosensors for carbohydrate analysis. Both sensors are based on resonance energy transfer between a donor and acceptor, which is affected by the presence of carbohydrates. The first sensor type is based on the use of luminescent semiconductor CdSe quantum dots (QDs)-dextran conjugates. Energy transfer between QDs of different size and emission color in the presence of carbohydrates is the basis for carbohydrate detection. The second approach for carbohydrate detection employs submicrometric phospholipid coated particles, named FRET trap lipobeads. To fabricate the FRET trap lipobeads fluorescent-labeled glucose binding lectin and Alexa 488 labeled phospholipids are incorporated into the phospholipid-coating layer of the lipobeads. Binding of acceptor labeled sugar based drugs to lectins leads to a FRET signal between the donor (Alexa 488) and the acceptor (Texas red). Both approaches provide viable alternative to electrochemical techniques used for carbohydrate detection.