IEC 162 |
| Rajendra K. Aithal1, Devendra S. Patel2, Gopal Krishna3, Yuri M. Lvov4, Tünde Mester5, Ming Tien5, and Debasish Kuila4. (1) Institute for Micromanufacturing, Louisiana Tech University, 911 Hergot Ave., Ruston, LA 71272, (2) Institute for Micromanufacturing/Chemical Engineering, Louisiana Tech University, Ruston, LA 71272, (3) Institute for Micromanufacturing/Biomedical Engineering, Louisiana Tech University, Ruston, LA 71272, (4) Institute for Micromanufacturing/Chemistry, Louisiana Tech University, Ruston, LA 71272, (5) Department of Biochemistry and Molecular Biology, Pennyslvania State University, University Park, PA 16807 |
| Most of the organic pollutants including phenolic and polycyclic aromatic hydrocarbons (PAHs) are bioaccumulative and highly toxic. Extracellular enzymes such as, Lignin Peroxidase (LiP) and Manganese Peroxidase (MnP) from P. chrysosporium are capable of degrading these pollutants efficiently in the presence of hydrogen peroxide. We have begun developing layer-by-layer (LbL) thin films of LiP and MnP with polycations to enhance and optimize its performance towards degradation of pollutants. The growth of the assembly is being monitored using AFM and a quartz crystal microbalance (QCM). The frequency shift of the QCM resonator increases with increase in thickness of the layers after each adsorption cycle. Our initial experiments on degradation of model compounds like veratryl alcohol and 2,6-dimethoxyphenol suggests that extracellular enzymes can be precisely deposited onto charged surfaces. The long-term stability of the LbL assembled enzymes, their structure and their activity towards degradation of aromatic and phenolic compounds will be presented.
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General Poster Session
5:00 PM-7:00 PM, Tuesday, March 25, 2003 Convention Center -- Hall G, Poster
Sci-Mix
Division of Industrial and Engineering Chemistry |