IEC 79 |
| Chad Mirkin, Department of Chemistry, Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113 |
| Multiplexed detection of oligonucleotide targets has been performed with gold nanoparticle probes labeled with oligonucleotides and Raman-active dyes. The gold nanoparticles facilitate the formation of a silver coating that acts as a surface-enhanced Raman scattering promoter for the dye-labeled particles that have been captured by target molecules and an underlying chip in microarray format. The strategy provides the high-sensitivity and high-selectivity attributes of gray-scale scanometric detection but adds multiplexing and ratioing capabilities because a very large number of probes can be designed based on the concept of using a Raman tag as a narrow-band spectroscopic fingerprint. Six dissimilar DNA targets with six Raman-labeled nanoparticle probes were distinguished, as well as two RNA targets with single nucleotide polymorphisms. The current unoptimized detection limit of this method is 20 femtomolar. |
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Nanoscale Sensing Technology: Why and How? (sponsored by Advanced Materials & Nanotechnology Subdivision)
2:00 PM-5:05 PM, Monday, March 24, 2003 Convention Center -- Room 394, Oral
Division of Industrial and Engineering Chemistry |