IEC 237 |
| Amanda J. Haes and Richard P. Van Duyne. Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208 |
| Triangular silver nanoparticles (~90 nm wide and 50 nm high) have remarkable optical properties. The enhanced sensitivity of the nanoparticles to their nanoenvironment has allowed us to develop a new class of optical nanosensors. Using localized surface plasmon resonance (LSPR) spectroscopy, the model system, biotinylated surface-confined Ag nanotriangles, was used to detect less than one picomolar up to micromolar concentrations of streptavidin. This response can be amplified by exposing the streptavidin-coated surface with biotinylated Au colloids. The system was vigorously tested for nonspecific binding interactions and was found to display virtually no adverse results. Additionally, the monitoring of anti-biotin binding to biotinylated Ag nanotriangles exhibited that the system could be used as a solution immunoassay. New improvements of the sensor design have increased its ruggedness. These results represent important new steps in the development of the LSPR nanobiosensor for applications in medical diagnostics, biomedical research, and environmental science. |
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Biological Applications of Nanomaterials and Nanotechnology (sponsored by Advanced Materials & Nanotechnology Subdivision)
8:00 AM-12:05 PM, Wednesday, March 26, 2003 Convention Center -- Room 393, Oral
Division of Industrial and Engineering Chemistry |