IEC 161 |
| Sathyajith Ravindran, Department of Chemical and Environmental Engineering, University of California, B-136 Bourns Hall, University of California, Riverside, Riverside, CA 92521, Sumit Chaudhary, Department of Electrical Engineering, University of California, B148 Bourns Hall, University of California, Riverside, Riverside, CA 92521, Mihri Ozkan, Departments of Electrical Engineering and Chemical and Environmental Engineering, University of California at Riverside, Bourns Hall A241, Riverside, CA 92521, and Cengiz Ozkan, Department of Mechanical Engineering, University of California, A305 Bourns Hall, University of California, Riverside, Riverside, CA 92521. |
| The chemical inertness of Carbon Nanotubes along with their nanoscale dimensions renders them as potential candidates for biological applications. Current research is underway to investigate the biocompatibility of carbon nanotubes by immobilization of biopolymers and proteins on the nanotubes. Here, we report the immobilization of transferrin tagged with a FITC fluorescent label on carbon nanotubes. The immobilization process takes place by a covalent amide bond formation between the oxygen containing groups on the nanotubes and the amines in the residues of the protein. 1–ethyl–3–(3–dimethylaminopropyl) carbodiimide HCl (EDC) in the presence of sulfo–N–Hydroxysuccinimide (sulfo–NHS) is utilized for this amide bond formation between the –COOH and the –NH2 groups. Characterizations using atomic force microscopy, thermal gravimetric analysis, Raman and confocal microscopy indicated the successful conjugation. Transferrin is an endocytosis mediating protein and this functionalization can be extremely promising in the in-vivo applications of carbon nanotubes. |
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General Poster Session
5:00 PM-7:00 PM, Tuesday, March 25, 2003 Convention Center -- Hall G, Poster
Sci-Mix
Division of Industrial and Engineering Chemistry |