IEC 249 |
| Paul Todd, Nathan A. Thomas, David J. Kennedy, Willliam V. Johnson Jr., Lara M. Deuser, and Heidi C. Platt. SHOT, Inc, 7200 Highway 150, Greenville, IN 47124 |
| Two problems in magnetic separations were addressed: the purification of manufactured particles according to net magnetic susceptibility and the sorting of biological cells in proportion to the number of magnetic particles bound. In multistage magnetic sorting a shallow sample cuvette receives magnetic particles and/or magnetic cells that are allowed to settle to the bottom. Then successively increasing magnetic fields are applied to attract the successively weaker particles upward into inverted, liquid-filled cavities, up to 15 in number, above the sample cuvette. Thus populations of magnetic particles and/or cells are subdivided into 15 subpopulations according to their magnetophoretic mobility. Commercial magnetic reagents, also biological cells, were separated into several fractions. By collecting and counting cells in the inverted cavities histograms of magnetophoretic mobility were obtained. The multistage magnetic sorter is fully automated and controlled by a graphical user interface. (Research supported by NASA and Indiana 21st Century Research and Technology Fund.) |
|
Magnetic-Field-Enhanced Separation and Related Processes (sponsored by Separation Science & Technology Subdivision)
9:00 AM-11:55 AM, Thursday, March 27, 2003 Convention Center -- Room 394, Oral
Division of Industrial and Engineering Chemistry |