IEC 196 |
| Barbara J. Panessa-Warren, Biology Department, Brookhaven National Laboratory, Building 463, Upton, NY 11973-5000, Stanislaus S. Wong, Department of Chemistry, SUNY at Stony Brook, Stony Brook, NY 11794, John B. Warren, Instrumentation Division, Brookhaven National Laboratory, Building 535B, p.o.Box5000, Upton, NY 11973-500, Berhane Ghebrehiwet, Dept. of Medicine, State University of New York at Stony Brook, School of Medicine, Stony Brook, NY 11793, and George T. Tortora, Clinical Microbiology Laboratory, State University of New York at Stony Brook, University Hospital, Stony Brook, NY 11793. |
| Single walled carbon nanotube bundles (SWNTs) were cleaned, cut, and formed into nanoloops, having a mean diameter of 29-55nm.With the use of standard biochemistry protocols, these nanoloops were functionalized with monoclonal antibodies to a human cell surface protein (gClq-R)involved with bacterial invasion. AFM and field emission SEM revealed specific localizations of the functionalized carbon nanoloops on Caco-2 cell microvilli adjacent to both bacterial cells and endospores. Cells not incubated with the microorganisms showed no spatial localization of the nanoloop probes, indicating that bacterial activation was necessary for directed surface expression of gClq-R protein. Conversely, nanoloops functionalized with a non-specific IgG, and pure gClq-R protein, and tested with bacteria-activated host cells, showed no binding. Therefore, immunologically functionalized carbon nanoloops can be used as a new tool in dynamic experiments to visually identify the presence of very specific cell surface proteins involved in microbial attack. |
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Biological Applications of Nanomaterials and Nanotechnology (sponsored by Advanced Materials & Nanotechnology Subdivision)
8:00 AM-12:05 PM, Wednesday, March 26, 2003 Convention Center -- Room 393, Oral
Division of Industrial and Engineering Chemistry |