IEC 197 |
| Jeff W.M. Bulte1, Ian D. Duncan2, Joseph A. Frank3, Trevor Douglas4, and Dara L. Kraitchman1. (1) Department of Radiology, Johns Hopkins University Medical School, 217 Traylor, 720 Rutland Ave, Baltimore, MD MD 21205, (2) Dept of Medical Sciences, University of Wisconsin, 2015 Linden Drive West, Madison, WI 53706, (3) Experimental Neuroimaging Section, National Institutes of Health, B10, B1N256, 10 Center Dr MSC 1074, Bethesda, MD 20892, (4) Department of Chemistry and Biochemistry, Montana State University, 108 Gaines Hall, Bozeman, MT 59717 |
| Superparamagnetic iron oxide nanoparticles are currently being used as MR contrast agents for imaging of phagocytosing cells in vivo. For magnetic labeling of non-phagocytes, these particles need to be modified in order to induce sufficient uptake. We have achieved a high degree of intracellular labeling by coating the particles with internalizing monoclonal antibodies, dendrimers, or poly-L-lysine. Following grafting of magnetically labeled oligodendrocyte progenitors in the spinal cord and brain of myelin-deficient rats, cell migration could be easily identified by MR imaging, with a good agreement between the areas of MR contrast enhancement, immunohistochemical staining for newly formed myelin, and staining for b-galactosidase expression. Furthermore, magnetically labeled mesenchymal stem cells, injected intramyocardially in a swine myocardial infarct model, could be clearly visualized and serially tracked over time. We conclude that MR imaging can non-invasively determine the biodistribution of cells following transplantation, setting the stage for clinical monitoring of cell-based therapies. |
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Biological Applications of Nanomaterials and Nanotechnology (sponsored by Advanced Materials & Nanotechnology Subdivision)
8:00 AM-12:05 PM, Wednesday, March 26, 2003 Convention Center -- Room 393, Oral
Division of Industrial and Engineering Chemistry |